This product equipped either with the Lucia camera or the Nikon DS-Qi2 camera bears the CE marking and can be also used as an IVD (in vitro diagnostic) medical device.

Automated FISH Image Acquisition

Image acquisition with different levels of user control, from fully-automatic to fully-manual

  • Control of all motorized parts of the microscope and its accessories

  • Image acquisition settings saved per fluorescent probe set

  • Eye-piece observation filter per fluorescent probe set

  • Intelligent automatic exposure control

  • Live image speed acceleration

  • Maximum-quality or minimum-time image acquisition.

  • Extended depth of focus (EDF) acquisition for thick samples such as tissue sections.

True or False Color Display

  • Each layer of the resulting image is associated with a specific probe. The system assigns a probe name and color to each layer.

  • The name and color of the layers can be changed at any time. This information is stored with the image.

  • True (real) or false (pseudo) color display can be achieved by assigning appropriate colors to each image component.

  • One component, usually DAPI, can be marked as counterstaining - this component can be displayed as inverted (black on the white background).

  • Any combination of components or a single component alone can be displayed.

  • The color display options can differ for on-screen display, reports and exports.

Image Enhancements and Annotations

  • To improve the contrast of individual components, simply specify a portion of the image as background .

  • Delete unwanted object with a simple eraser.

  • Tune the resulting colors by the signal purification procedure or by color unmixing.

  • Align components by pixel shifting to correct possible inaccuracies caused by misalignment of filter cubes.

  • Simply annotate the image using custom predefined phrases and arrows.

Preimplantation Genetic Diagnosis (PGD)

is the genetic testing of preimplantation stage embryos for specific single gene disorders or heritable chromosomal imbalance. PGD for gender selection, aneuploidy, and structural abnormalities involves the biopsy of one or both polar bodies or the biopsy of one or two blastomeres (cells from 2 to 16 cell stage embryos), fixation to glass slides, followed by fluorescence in situ hybridization (FISH). One of the most critical steps in PGD is the fixation required to obtain good FISH nuclear quality without losing any of the cells analyzed.

For more information see Preimplantation Genetic Diagnosis (PGD) .

Integration with Other LUCIA Products

Program LUCIA Cytogenetics - FISH is fully compatible and can be integrated with other LUCIA products: