LUCIA Cytogenetics is able to visualize the entire genome in multiple colors.
LUCIA Cytogenetics can work with fluorescent probe kits from different manufacturers.
Get high-quality images with scientific digital CCD cameras with 14bits analog-to-digital conversion.
The automatic camera exposure control ensures that maximum information is transferred to the computer, no detail gets lost.
The complete control over a motorized microscope speeds up the image acquisition and reduces the risk of human-caused errors.
Predefined acquisition settings for various probe kits.
See any combination of the individual color images composed into one.
See the rearrangements in pseudo color from the acquired image to the karyogram.
Labeling scheme indicators will always help you to stay informed and understand each color stain.
Automatic color classification including fast pseudo color display on the raw image.
Automatic and manual spot filtering.
Rearrangement analysis allows to quickly show only the material from the selected chromosomes and thus helps clearly reveal the rearrangement.
Profile graphs allow to study the intensity profiles for all colors.
The multiplex fluorescence in situ hybridization (MFISH) method uses a series of optical filters to image each of the five fluorophores, applies the LUCIA Cytogenetics program to merge the images into a composite in which each chromosome is assigned a distinct color based on its fluorophore composition.
MFISH complements the standard cytogenetic methods. It is very helpful in deciphering complex chromosomal rearrangements and is used to identify non-random structural chromosome rearrangements not detectable by other methods.
MFISH allows rapid identification of simple and complex chromosomal alterations in metaphase spreads which may be associated with a disease initiation and progression. Therefore the applications of MFISH imaging are spreading fast - particularly in the field of cancer research.
Since M-FISH (24-color FISH) is based on chromosome painting it can only be applied to metaphases.