Lucia Cytogenetics

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LUCIA Cytogenetics™

LUCIA Cytogenetics™ is able to visualize the entire genome in multiple colors.

The multiplex fluorescence in situ hybridization (MFISH) method uses a series of optical filters to image each of the five fluorophores, applies the LUCIA Cytogenetics™ program to merge the images into a composite in which each chromosome is assigned a distinct color based on its fluorophore composition.

MFISH complements the standard cytogenetic methods. It is very helpful in deciphering complex chromosomal rearrangements and is used to identify non-random structural chromosome rearrangements not detectable by other methods.

MFISH allows rapid identification of simple and complex chromosomal alterations in metaphase spreads which may be associated with a disease initiation and progression. Therefore the applications of MFISH imaging are spreading fast - particularly in the field of cancer research.

Since M-FISH (24-color FISH) is based on chromosome painting it can only be applied to metaphases.

LUCIA Cytogenetics™ can work with fluorescent probe kits from different manufacturers.

Fast and easy image acquisition

  • Get high-quality images with scientific digital CCD cameras with 14bits analog-to-digital conversion.

  • The automatic camera exposure control ensures that maximum information is transferred to the computer, no detail gets lost.

  • The complete control over a motorized microscope speeds up the image acquisition and reduces the risk of human-caused errors.

  • Predefined acquisition settings for various probe kits.

See all details in full spectrum

  • See any combination of the individual color images composed into one.

  • See the rearrangements in pseudo color from the acquired image to the karyogram.

  • Labeling scheme indicators will always help you to stay informed and understand each color stain.

Utilize all information using the analysis tools

  • Automatic color classification including fast pseudo color display on the raw image.

  • Automatic and manual spot filtering.

  • Rearrangement analysis allows to quickly show only the material from the selected chromosomes and thus helps clearly reveal the rearrangement.

  • Profile graphs allow to study the intensity profiles for all colors.

Database and Report

  • Fully compatible with LUCIA Cytogenetics™ Database.

  • Fully compatible with LUCIA Cytogenetics™ Report Tool including all MFISH display options.