Lucia Cytogenetics

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FISH

LUCIA Cytogenetics™


Automated Microscope Control

LUCIA Cytogenetics™ is automation-friendly:

  • Control of all motorized parts of a microscope

  • Control of external devices: shutter, filter turret, filter wheels, light path etc.

  • Image acquisition settings per fluorescent probe set

  • Observation filter (used for eye piece) per fluorescent probe set

  • Z-axis control.

Flexible FISH Image Acquisition

Image acquisition with different levels of user control, from fully-automatic to fully-manual

  • Separate camera settings are saved for each fluorescent probe.

  • Various automatic exposure options

    • Continuous or single-push auto exposure

    • User defined target values (% of overexposed pixels, target maximal intensity)

    • Live image speed acceleration

    • Reducing image to a region of interest (area probe)

  • Usage of a highly sensitive CCD camera (up to 14 bits and 67 dB SNR).

  • Maximum-quality or minimum-time image acquisition.

  • Extended depth of focus (EDF) acquisition for thick samples such as tissue sections.

  • Fully automated EDF.

Thick sample acquisition

  • Different objects are visible when focusing through Z axis (Z1, Z2).

  • The thick sample acquisition (EDF) tool merges focused areas of the whole Z-stack and creates a single perfect image.

True or False Color Display

  • During the acquisition, each image component is associated with a probe and obtains its name and display color.

  • The name and color of the components can be changed later, the information is stored with the image.

  • True (real) or false (pseudo) color display can be achieved by assigning appropriate colors to each image component.

  • One component, usually DAPI, can be marked as counterstaining - this component can be displayed as inverted (black on the white background).

  • Any combination of components or a single component alone can be displayed.

  • The color display options can differ for on-screen display, reports and exports.

The counterstaining component displayed as inverted

True color report

Pseudo color report

True color display

Thick sample captured with LUCIA Cytogenetics

Blastomere

Image enhancements and Annotations

  • Easy per component contrast enhancement can be done by specifying part of the background in the image.

  • Delete unwanted object with a simple eraser.

  • Tune the resulting colors by the signal purification procedure or by color unmixing.

  • Align components by pixel shifting to correct possible inaccuracies caused by misalignment of filter cubes.

  • Simply annotate the image using custom predefined phrases and arrows.

    Drag-and-drop annotation tool

Database and Report

  • Use the powerful Lucia Database™ to store and manage your results.

  • Create perfectly looking layouts for reports, presentations or publications.

  • Modify the image display specially for report purposes - e.g. a selected component, any component combination, inverted counterstain, thumbnail mosaic.

Print-optimized report, mosaic

Image / patient database