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PGD

LUCIA FISH in Preimplantation Genetic Diagnosis

Description of methods:

Fluorescence in situ hybridization (FISH) is a technique that allows visualization of specific nucleic acid sequences (specific chromosomal regions) within a cellular preparation. FISH involves the precise annealing of a single stranded fluorescently labeled DNA probe to complementary target sequences (to chromosomes or nuclei). The hybridization of the probe with the cellular DNA site is visible by direct detection using fluorescence microscopy.

Preimplantation genetic diagnosis (PGD) is the genetic testing of preimplantation stage embryos for specific single gene disorders or heritable chromosomal imbalance. PGD for gender selection, aneuploidy, and structural abnormalities involves the biopsy of one or both polar bodies or the biopsy of one or two blastomeres (cells from 2 to 16 cell stage embryos), fixation to glass slides, followed by fluorescence in situ hybridization (FISH). One of the most critical steps in PGD is the fixation required to obtain good FISH nuclear quality without losing any of the cells analyzed.

Fig. 1
PGD on normal male blastomera.
Red: LSI 21,
Green: LS I 13,
Blue: CEP 18,
White: CEP Y,
Yellow: CEP X.
Courtesy of O. Scheinost, Dept. of Medical Genetics, Hospital České Budějovice.


Introduction

Numerical chromosome abnormalities are the major cause of inherited diseases with an incidence of 21% in spontaneous abortions. Of these, trisomies for gonosomes and chromosomes 21, 18, 16 and 13 account for 50% of chromosomaly abnormal abortions. Because of the correlation between aneuploidy and declining implantation rates with maternal age it was postulated that the screening of chromosome aneuploidies in human embryos by FISH using X, Y, 18,13 and 21 probes should significantly reduce the risk of older IVF patients delivering trisomic offspring (Munne S., 2002). Currently, negative selection of aneuploid embryos can only be done through PGD. Conventional techniques for cytogenetic analysis usually depend on visualizing chromosomes in metaphase. This is not possible at prei mplantation stage as a reliable karyotype cannot be obtained by spreading just one or two cells. FISH allows chromosome enumeration to be performed on interphase cell nuclei, i.e. without the need for culturing cells or preparing metaphase spreads. FISH has been applied to PGD of common aneuploidies using human blastomeres (or oocyte polar bodies). Mosaicism cannot be detected efficiently by PGD unless all cells lines are abnormal.


Laboratory Routine

MultiVysion® PGT Multi-color Probe a MultiVysion® PB Multi-color Probe, two complementary DNA FISH reagents, are designed for detection of chromosome copy number in single cells such as human blastomere cells and polar body (DNA extruded from the ovum prior to and immediately following fertilization). MultiVysion PGT consists of a 5 probes with sequences homologous to specific regions on chromosomes 13,18, 21, X and Y, MultiVysion PB consists of probes for chromosomes 13, 16, 18, 21 and 22. Those reagents consist of DNA probes specific to chromosomes that are frequently identified with abnormal copy number in polar bodies and in abnormal embryos, prior to implantation for IVF and can detect the most common chromosome abnormalities leading to spontaneous miscarriages and the birth of affected offspring. Many laboratories investigate in their PGD program routinely chromosomes 13, 18, 21, X,Y (e.g. Vysis MultiVysion PGT) in human blastomeres. Routine investigation of chromosomes 16 and 22 in human blastomeres is also very common and profitable. Screening the chromosomes of preimplantation embryos using PGD techniques allows embryos with a high developmental potential (normal number of chromosomes) to be detected and used for IVF in preference to aneuploid embryos, thus increasing pregnancy rates.


LUCIA contribution in evaluation

LUCIA FISH workstation can facilitate capturing, evaluation and documentation of multi-color fluorescence images in PGD remarkably. LUCIA FISH system includes a high sensitive monochrome digital camera that provides superb image quality and minimal signal fading. The system enables to utilize capturing procedure including the microscope automation. The displaying brings the highest flexibility possible. User can define number of color channels, to name them and to specify their display colors. Additionally the way how the channels are mixed together can be selected.

Fig. 2
MultiVysionTM PGT probe mixture consists of
LSI 13 SpectrumRed,
LSI 21 SpectrumGreen,
CEP 18 SpectrumAqua,
CEP X SpectrumBlue,
CEP Y SpectrumGold.
The blastomere was stained with DAPI (displayed in gray).


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